ANTIBACTERIAL ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACTS OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON SOME BACTERIA ISOLATES

ABSTRACT Antibacterial activities of Thymus vulgarisand Myristicafragrans seeds on some bacteria isolates was studied. Different concentrations (90%, 70%, and 50%) of the selected plant extract ofThymus vulgarisand Myristicafragrans seedswere prepared. The present study shows that the highest bacterial count was from wound swab A with 71 Cfu/cm3, followed by wound swab C with 52 Cfu/cm3, then wound swab B with 46 Cfu/cm3, then wound swab E with 31Cfu/cm3 and the least wound swab D with 22Cfu/cm3. The cultural, microscopic and biochemical characterization of the bacterial isolates revealed three (3) isolates such as: Staphylococus sp,Pseudomonas sp and Proteus sp. The highest prevalence was fromStaphylococcus spwith 35.5%; followed by Pseudomonas sp with 34.3% and the least was Proteus sp with 30.2% respectively. The effect of selected plant extract on the growth of the bacterial isolates revealed the plant extracts of Myristicafragrans seedsat 90%, 70% and 50% concentrations was observed to have a growth inhibition on Staphylococcus spas 11mm for 70% concentration for ethanolic extract and 11mm, 13mm, 9mm for aqueous extract.Pseudomonas sp had zones of inhibition of 10mm at 90% concentration for ethanolic extract and 9mm, 11mm, 10mm for aqueous extract. Proteus sp had zone of inhibitions of 11mm, 10mm at 90%, 70% concentration for ethanolic and 10mm, 10mm, 8mm for aqueous extracts. While the plant extracts of Thymus vulgaris seedsat 90%, 70% and 50% concentrations was observed to have a bacterial growth inhibition on Staphylococcus spas follows 13mm, 15mm, 12mm for ethanolic and 16mm, 14mm and 13mm for aqueous extracts, Pseudomonas sp had zones of inhibitions of 8mm at 50% concentration for ethanolic and 7mm, 9mm and 9mm for aqueous extracts.Proteus sp showed negligible response at all concentrations for ethanolic and 9mm, 8mm and 8mm for aqueous extracts respectively.