A PROJECT REPORT SUBMITTED TO THE DEPARTMENT OF BIOCHEMISTRY ON THE TOPIC SERUM ALKALINE PHOSPHATASE LEVEL OF ALBINO RAT TREATED WITH ETHANOL STEM EXTRACT OF COSTUS AFER.
Abstract
Abia state university, uturu.
Acknowledgment
I will always remain grateful to my supervisor Prof G. C Chinyere for his professional guidance to ensure an efficient work production . And I also want to thank sir chinedu for his help when called upon. I wont fail to recognize all my distinguished lecturers Prof E.I Akubugwo, Prof F.O uhaegbu , Prof G.C Chinyere, Dr. J.C Nwaogugwo, Mr. O.C Atasie,and Mr. Okezie Emmanuel for their various knowledge impartation.
I acknowledge my family members for their support financially,academically and otherwise to ensure a successful outcome of this work.
ABSTRACT
This research is designed to study the effect of ethanol stem extract of costus afer on female albino rats after 15 days of treatment. The animals were divided into 3 groups of six (6 ) animals each and each of the group received standard feed and water. Group 1 served as the control, while Group 2 served as the low dose and was administered 400mg/kg.Bw of the stem extract and group 3 served as the high dose and was also administered 800mg/kg.BW of the stem extract. The animals weight were monitored daily and it was observed that there was an increase in the body weight of each of the animals in the test gropus. The effect of costus afer on the liver biomarkers, Alkaline phosphatase, Lipid biomarkers,such as Triacylglycerol,Total cholesterol, and renal biomarkers such as creatinine, urea,sodium,and chlorine were investigated. Results showed that in the liver function test, ALP showed a significant increase ( p 0.05) when compared to the control at day 5 and only TC and HDL showed a significant increase at both high and low dose in day 15.
Table 4A
Effect of ethanol stem extract of costus afer on the molecular weight of female albino rats after 5 days of treatment.
Treatment Molecular Weight
G1( control) 65.16 ± 5.50
G2(LD) 400mg/kgb.w 64.69 ± 8.65
G3(HD) 800mg/ kgb.w 72.10 ± 6.43
Table 4B
Effect of ethanol stem extract of costus afer on the molecular weight of female albino rats after 10 days of treatment.
Treatment Molecular Weight
G1( control) 74.13 ± 7.40
G2(LD) 400mg/kgb.w. 78.34 ± 7.81
G3(HD) 800mg/kgb.w 79.45 ± 6.30
Table 4C
Effect of ethanol stem extract of costus afer on the molecular weight of female albino rats after 15 days of treatment
Treatment Molecular Weight
G1( control) 88.68 ± 12.96
G2( LD) 400mg/ kgb.w 97.69 ± 16.37
G3(HD) 800mg/ kgb.w 99.70 ± 11.45
From the table above result show that there was a significant increase( P
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