THIN LAYER CHROMATOGRAPHY OF MORINGA OLIFERA AND AZADIRACHTA INDICA LEAVES EXTRACTS
Abstract
ABSTRACT
Moringa Oleifera and Azadirachta indica are both useful traditional plant growing in
Nigeria, India and America. The thin layer chromatography is a simple, quick analytical
tool used to separate, identified different bioactive compound such as flavonoids, Tannins,
phenolics, Alkaloids and saponins. The plant material was extracted using methanol, ethyl
acetate and n-hexane. The Rf value of different solvent ratio were calculated for each
extract ; Moringa & Neem N-Hexane: ethyl acetate 3:1 0.33-0.85 & 0.62-0.95 , NHexane 3 0.87 & 0.12-0.96 , N-Hexane : Chloroform 3:1 0.27-0.68 & 0.10-0.97. The
results show that different functional group were observed due to the IR spectroscopy at
different frequency ( cm-1) such as 2928 for Alkane C-H , 2868 for Aldehyde C-H , 1745
for Ester C=O , 1629 for Alkene C=C , 3332 for Alcohol OH ,1508 for Aromatic C=C , 3555 for
Amine N-H , 1670 for Amides C=O , 873 for Aromatic C-H , 1655 for ketone (conj) C=O , 2112
for Alkynes , 672 for Aromatic C=C-H , 1719 for Carboxylic acid C=O , 1557 for Nitro ( RNO2 )
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