PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL EFFICACY OF SESBANIA BISPINOSA (ZAMARKE) AGAINST CLINICAL ISOLATES
Abstract
This study investigates the phytochemical screening and antibacterial efficacy of Sesbania
bispinosa against clinical isolates of Staphylococcus aureus (Gram-positive) and Escherichia
coli (Gram-negative). The plant, commonly used in traditional medicine, was subjected to a
range of chemical tests to identify its bioactive compounds. The phytochemical analysis
revealed the presence of alkaloids, flavonoids, saponins, glycoside, anthraquinones, steroid,
terponoid, tannins, and phenolic compounds, all of which are known for their antimicrobial
properties. The antibacterial activity was evaluated using the disc diffusion method, against
Staphylococcus aureus and Escherichia coli. The antibacterial activity of the extracts was
monitored at different Concentratios of 100mg/ml, 50mg/ml, and 25mg/ml prepared from the
stock concentration(100mg/ml) by serial dilution with standard drug: Amoxicilin. The
antibacterial activity of ethanolic extract of Staphylococcus aureus at Concentration of
100mg/ml, showed inhibition of 19.6±4.16 at 50mg/ml 17.6 ± 1.15 and 25mg/ml The zone of
inhibition showed 19.6 ± 4.16. across positive control 31.3 ± 30. Escherichia coli zone of
inhibition at Concentration of 100mg/ml, showed 58.6 ± 74.7, 50mg/ml 18.3 ± 0.57, 25mg/ml
the zone of inhibition showed 20.6 ± 4.04, across positive control 28.3 ± 1.52 respectively. The
Aqueous activity of extract Staphylococcus a
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