CHROMATOGRAPHIC ANALYSIS OF HIBISCUS SABDARIFFA (ROSELLA) AND VERNONIA AMYGDALINA (BITTER LEAF) LEAF EXTRACTS AND LETHALITY CONCENTRATION USING BRINE SHRIMP (ARTEMIA SALINA)

ABSTRACT This project is aimed at isolation and identification of phytochemical extracts from plant materials of Rosella H. sabdariffa and V. amygdalina,, and it is essential in understanding their potential biological activities. This study outlines a detailed methodology for the evaluation of chromatography (thin layer and column) and lethality concentration analysis of H. sabdariffa and V. amygdalina, leaves extract against Artemia salina (brine shrimp). It is imperative to characterize these extracts using chromatographic techniques and to assess their toxicity levels through lethality concentration (Lc50) determination. The analysis is instrumental in evaluating the promising bioactive compounds found in these plants and their potential applications in biomedicine and pest management. The methodology detailed herein is supported by recent literature and developments in phytochemical research. The study shows that Vernonia amgydline (717.27) is more toxic than hibiscus sabdariffa (856.92) overall based on the mortality rates and LC50 values as indicated in table 6. Both compounds exhibit decreased toxicity at lower concentrations, highlighting the need for careful assessment of exposure levels in environmental or practical applications. Studies could include longer exposure periods, behavioral observations, or tests with varying species to better understand the ecological impact of these compounds. Further research utilizing the brine shrimp lethality assay would be beneficial to comprehensively assess the cytotoxic potential of H. sabdariffa extracts.