EVALUATE THE IMPACT OF CULTURAL DRESSING AS A FORM OF RESISTANCE AND IDENTITY ASSERTION, FOCUSING ON EDO CULTURAL DRESSING.
Abstract
Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder characterized by persistent hyperglycemia, oxidative stress, and progressive damage to vital organs such as the liver. The search for effective, safe, and affordable alternatives to conventional therapies has led to growing interest in medicinal plants such as Nigella sativa (NS). This study evaluated the protective effects of Nigella sativa oil (NSO) against oxidative stress and histological alterations in the liver of type 2 diabetic male Wistar rats.
Twenty-five rats were divided into five groups: control, diabetic untreated, diabetic treated with metformin, and diabetic groups treated with NSO at doses of 0.5 mL/kg and 1 mL/kg. Diabetes was induced by fructose feeding and streptozotocin (STZ) injection. After four weeks of treatment, liver tissues and serum were analyzed for oxidative stress biomarkers including malondialdehyde (MDA), oxidized glutathione (GSSG), superoxide dismutase (SOD), catalase (CAT), and total thiols. DNA fragmentation index (DFI) and histological changes were also assessed.
Results showed that diabetes significantly elevated pro-oxidant markers (MDA, GSSG, DFI) and reduced antioxidant defenses (SOD, CAT, thiols), indicating severe oxidative stress and hepatic injury. NSO treatment markedly reversed these effects in a dose-dependent manner, with the 1 mL/kg group showing superior efficacy. NSO reduced lipid peroxidation, restored glutathione balance, and enhanced antioxidant enzyme activities—sometimes surpassing control levels. Histology confirmed improved liver architecture in NSO-treated groups.
In conclusion, Nigella sativa oil demonstrated potent antioxidant and hepatoprotective properties in diabetic rats, comparable to metformin. These findings support its therapeutic potential as a natural adjunct for managing diabetes-related liver complications and warrant further clinical evaluation.
Keywords: Type 2 Diabetes Mellitus, Oxidative Stress, Nigella sativa Oil, Hepatoprotection, Antioxidant Enzymes, Malondialdehyde, Glutathione.
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