COMPARATIVE STUDY OF ANTIBIOTICS RESISTANCE PATTERN OF ISOLATES FROM COMMERCIAL AND FREE RANGE POULTRY FARM WITHIN IKOT EKPENE

Student: Hossana Inemesit Okon
Supervisor: Mrs Rachael Udo
HOD: Mrs Imaobong Adenugba
Department of Science Laboratory Technology
School of Applied Science
AKWA-IBOM STATE POLYTECHNIC (IEI), IKOT-OSURUA, AKWA IBOM STATE

Abstract

The comparative study of antibiotics resistance pattern of isolates from commercial and free range poultry farm within Ikot Ekpene was carried out using standard bacteriological techniques. The results of the study revealed that droppings from CPFA recorded highest bacterial counts of (1.30 x 106cfu/g), CPFB had bacterial counts of (1.20 x 106cfu/g), BPFA recorded bacterial counts (1.00 x 106cfu/g), BPFB) recorded bacterial counts (9.0 x 105cfu/g). The study revealed the presence of six bacteria genera to include Enterococcus sp., Staphylococcus sp., Escherichia sp., Salmonella sp, Campylobacter sp. and Klebsiella sp. Escherichia sp recorded the highest frequency and percentage occurrence 151(34.3%), Salmonella sp 102 (23.2%), Enterococcus sp had 93(21.1%), Staphylococcus sp 37(8.4%), Campylobacter sp had 35(8.0%), while Klebsiella sp recorded the least frequency and percentage occurrence of 22 (5.0%). The antibiotic susceptibility pattern of Gram positive and negative bacterial against commercial sensitivity disc revealed that Staphylococcus sp was sensitive to ciprofloxacin with zone of inhibition (28mm), Enterococcus sp was sensitive to levofloxacin (27mm), erythromycin (26mm), streptomycin (19mm) and ciprofloxacin (17mm), Salmonella sp was sensitive to cefuroxim (28mm), perfloxacin (21mm), Ofloxacin and Ceftazidime (19mm) each, Escherichia sp was sensitive to ciprofloxacin (25mm), Ceftazidime (24mm) and perfloxacin (21mm), while Campylobacter sp and Klebsiella sp recorded no sensitive responses to any of the tested antibiotics. Poultry farms could be a potential source of resistant bacterial, which could be detrimental to human when transferred to human through infected plants products.

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