IDENTIFICATION OF BACTERIA ASSOCIATED WITH DIABETIC FOOT ULCERS IN PATIENTS ATTENDING ABIA STATE UNIVERSITY TEACHING HOSPITAL, ABA.

Student: Amarachi Olanma Okwara
Supervisor: Dr Nwachukwu O. Ndubuisi
HOD: Dr Okezie Caleb Okamgba
Department of Medical Laboratory Science
Medical Health Science
Abia State University, Uturu, Abia State

Abstract

ABSTRACT Diabetic ulcers (DFUs) are a common complication of diabetes, particularly in individual with uncontrolled or poorly managed blood sugar levels, thus representing a major cause of lower limb amputation worldwide. Bacterial organisms such as Staphylococcus species, Kelebsiella Species, Pesuodomonas, aeruginosa etc colonize the surface wound which provides an ideal which for future invasion resulting to infection. Studies may have show effective management of DFU and prevention measure to have taken place in north America Europe but not much of it has not been experienced in Africa especially the current study area Absuth, Aba, Abia state The aim of this study was to determine bacteria associated with diabetic foot ulcer in patients in Absuth, Aba. A hospital based study involving DFU patients attending clinics at Absuth, Aba , Abia state Nigeria city in the south East Nigeria was implemented. A total of 30 wound swab using a sterile swab stick were collected, from diabetic foot ulcer patients. The use of Blood agar, Macconkey agar were utilized to culture the samples for growth of microorganisms. Each bacterial colony on the agar plates were observed for morphological characteristics such as size shape Gram staining and biochemical test such as catalase test, indole test etc was used to characterize and identity the bacterial isolation. The samples (80%) showed a significant growth and (20%) showed some growth , significant growth was observed mostly in make (80%) than female (20%) aged 40-49 this study showed the effective antibiotics against the isolates, Amoxicillin and Streptomycin, and the less effective antibiotics, Gentamicin, Ampiclox and Pefloxacin which was carried out using KIRBY — BAUER SUSCEPTIBILITY TECHNIQUE. Diabetic foot ulcer can lead to serve consequences if not presented early pr properly treated, this study highlights the critical need for early detection proactive management and effective prevention strategies for DFUs. This study could serve as future referencing on research of DFUs, ultimately improving the outcome for these are risk of diabetic foot ulcers.

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