EFFECT OF GLIBENCLAMIDE ON TOTAL PROTEIN AND CHOLESTEROL LEVELS IN ANIMALS EXPOSED TO ACUTE PARACETAMOL INDUCED TOXICITY

ABSTRACT Background: The background of the study is the effect of glibenclamide on paracetamol-induced toxicity. Paracetamol (acetaminophen) is a widely used over-the-counter analgesic and antipyretic drug. While generally considered safe when taken at recommended doses, paracetamol overdose or prolonged use can lead to hepatotoxicity and other adverse effects. Paracetamol-induced liver injury is a major cause of acute liver failure worldwide. Glibenclamide is an oral sulfonylurea drug used primarily in the treatment of type 2 diabetes mellitus. Methods: The experimental animals were grouped into four groups of five animals each where; Group 1 served as normal control (10 ml/kg) daily, while groups 2 were administered paracetamol 500 mg/kg daily for 14 days to induce liver toxicity. Group 3 were administered paracetamol and glibenclamide (GLB) 5 mg/kg. Group 4 were administered silymarin (SLY) 100 mg/kg daily respectively for 14 days, using distilled water as the vehicle. At the end of the experimental period, the rats were anaesthetize using ether and blood samples were collected for analyses. Results: The study showed that paracetamol induced toxicity in the paracetamol control group animals as shown by a significant reduction in total protein and cholesterol level when compared to normal control group. Following treatment with glibenclamide and silymarin there was no significant difference in glibenclamide treated group. However silymarin showed a positive significant difference relative to paracetamol control group. Conclusion: in this study, it was determined that glibenclamide produced no significant effect in paracetamol induced toxicity