STUDYING THE OXIDATIVE STRESS RESPONSES IN KIDNEY OF RAT INDUCED BY SUBCHRONIC ORAL ADMINISTRATION OF POLYHERBAL INFUSION

ABSTRACT This study investigated the oxidative stress responses in the kidneys of rats following subchronic oral administration of a polyherbal infusion. The infusion, comprising green tea, black tea, dried clove, and ginger leaves, was administered to evaluate its effects on oxidative stress markers and renal function. Levels of malondialdehyde (MDA) were measured to assess oxidative damage, while superoxide dismutase (SOD) and catalase (CAT) activities were determined as indicators of antioxidant defense. Results indicated that the control group exhibited an MDA level of 0.10±0.01 nmol/mg protein, while various formulations showed significantly altered MDA levels, suggesting differential oxidative stress responses. The highest SOD activity was recorded in formulation F2 (0.71±0.06 U/mg protein), indicating enhanced antioxidant defense, whereas formulation F4 displayed the lowest SOD activity (0.46±0.06), correlating with increased oxidative stress. Notably, catalase activity was markedly elevated in the experimental groups compared to controls, with formulation F5 achieving the highest level (6.19±1.85 U/mg protein). These findings highlight the potential of polyherbal infusions to modulate oxidative stress and enhance renal antioxidant defenses. The study advocates for further research into the implications of polyherbal treatments on kidney health and their application in managing oxidative stress-related conditions.