THE EFFECT OF SUPPLEMENTING SUBSTRATES WITH NITROGEN SOURCES ON THE GROWTH AND YIELD OF PLEUROTUS PULMONARIUS

Student: Sophiat Oluwadamilare Jubril
Supervisor: Prof Oluseyi Damilola Adejoye
HOD: Prof A A Awofola
Department of Biology Education
College of Science and Information Technology
Tai Solarin Federal University of Education, Ijagun, Ogun State

Abstract

Pleurotus pulmonarius is a widely cultivated oyster mushroom valued for its nutritional composition and therapeutic potential, including its proteins, dietary fiber, essential minerals, and bioactive antioxidant compounds. Hence, this study examined the influence of nitrogen-enriched substrates on the growth and yield of Pleurotus pulmonarius. The experiment was conducted in the Department of Biological Sciences, Tai Solarin University of Education, Ijagun, using sawdust and rice husk sourced from Ijebu-Ode and Abeokuta, respectively, while spawns were obtained from the Federal Institute of Industrial Research (FIIRO), Oshodi. Prepared substrates were inoculated, and mycelial development was monitored weekly. Results showed clear variations among treatments. For fresh weight, T9 (150g of Ricehusk + 50g of Sawdust), T7 (150g of Sawdust + 50g of Ricehusk + 10g of Bloodmeal), and T5 (150g of Sawdust + 50g of Ricehusk + 10g of Fishmeal) were the top performers. In stalk length, T14 (200g of Ricehusk) and T5 (150g of Sawdust + 50g of Ricehusk + 10g of Fishmeal) ranked highest, while T5 also produced the largest cap diameter. Mycelial colonization followed a different pattern, with T13 (200g of Sawdust), T5, and T1 (100g of Sawdust + 100g of Ricehusk + 10g of Fishmeal) showing the fastest spread. These patterns indicate that rapid colonization does not necessarily correspond to superior yield. Overall, nitrogen supplementation improved growth and productivity. Substrates enriched with organic nitrogen sources supported earlier pinhead formation and higher yields than the control. Hence, nitrogen supplements should be added moderately to maintain a balanced carbon-to-nitrogen (C: N) ratio. Therefore, moderate organic nitrogen supplementation is recommended. KEYWORDS: Pleurotus pulmonarius, nitrogen, fishmeal, agricultural waste. Word count: 257

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