ANTIMICROBIAL ACTIVITY OF CHLORINE DISINFECTANT AGAINST CLINICAL ISOLATE OF STAPHYLOCOCCUS AUREUS

This study investigated the antimicrobial activity of chlorine disinfectants, specifically Dettol and Tetmosol, against clinical isolates of Staphylococcus aureus. Different types of disinfectants that are frequently employed in regular laboratories includes Dettol and Tetmosol. This study used the agar well diffusion method to evaluate the antibacterial effectiveness of two disinfectants namely Dettol and Tetmosol against Staphylococcus aureus at different concentrations. Dettol and tetmosol were obtained from Titus west supermarket Makurdi, Benue State. The Staphylococcus aureus used in this study was a clinical isolate obtained from wound swab samples collected. 100 ml of sterile, distilled water was combined with 27 g of Muller Hinton Agar, and the mixture was autoclaved at 121° C for 15 minutes. Antibiotic susceptibility test of the isolated test organisms against commonly prescribed antibiotics was determined using standard microbiological protocol. The results demonstrated that all the disinfectants in their concentrated forms prevented the growth of the test organism. The Inhibition activity of Staphylococcus aureus isolated from Clinical sample revealed that Dettol had a 33mm IZD and Tetmosol had 24mm IZD activity against the extract (Disinfectant) used. When different dilution of the disinfectants was used the zone of inhibitions varied as with different dilution. These findings revealed that Dettol had a better activity against Staphylococcus aureus than Tetmosol. Based on the outcomes of this study, it was recommended that, disinfectants such as Dettol and Tetmosol should be used in concentrated or minimally diluted forms, especially in healthcare settings where microbial contamination risk is high.