DETECTION OF EXTENDED SPECTRUM BETA-LACTAMASE (ESBL) PRODUCTION IN STAPHYLOCOCCUS AUREUS ISOLATED FROM URINE SAMPLES OF CATHETERIZED PATIENTS IN MAWC HOSPITAL SOKOTO METROPOLIS

ABSTRACT Catheter-associated urinary tract infections (CAUTIs) are among the most common hospital-acquired infections, particularly in patients with indwelling urinary catheters. The emergence of extended spectrum beta-lactamase (ESBL)–producing Staphylococcus aureus has further complicated the management of these infections due to increased antimicrobial resistance. This study was conducted to detect ESBL production in Staphylococcus aureus isolated from urine samples of catheterized patients attending Maryam Abacha Woman and Children Hospital, Sokoto. A cross-sectional laboratory-based study was carried out using urine samples collected aseptically from catheterized patients who had been catheterized for more than 48 hours. Isolation and identification of Staphylococcus aureus were performed using standard microbiological and biochemical techniques. Antibiotic susceptibility testing was carried out using the Kirby–Bauer disk diffusion method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. Detection of ESBL production was done using the Double Disk Synergy Test (DDST). All isolates recovered were identified as Staphylococcus aureus, representing a prevalence of 100%. The isolates showed high resistance to most beta-lactam antibiotics and several commonly used antimicrobial agents, but remained susceptible to rifampicin and gentamicin. The findings indicate the presence of ESBL-producing Staphylococcus aureus among catheterized patients. Routine ESBL screening and antibiotic susceptibility testing are recommended to guide effective treatment and enhance infection control practices.