PRODUCTION OF ANTIBACTERIAL FROM ALOEVERA PLANT AGAINST SELECTED BACTERIA (CLINICAL ISOLATE)

This study investigated the production of antibacterial agents from Aloe vera and their efficacy against selected clinical bacterial isolates (Escherichia coli and Staphylococcus aureus). Fresh Aloe vera leaves were collected from Rijiyar Zaki, Kano State, and authenticated at Bayero University Kano (BUK). The gel was extracted, concentrated, and subjected to phytochemical screening, revealing the presence of alkaloids, saponins, tannins, flavonoids, phenols, glycosides, quinones, anthraquinones, steroids, and terpenoids—compounds known for their antimicrobial properties. The antibacterial activity was evaluated using the agar well diffusion method at varying concentrations (100 mg/ml, 50 mg/ml, and 25 mg/ml). The results demonstrated significant inhibitory effects, with S. aureus showing higher susceptibility (zones of inhibition: 20–28 mm at 100 mg/ml) compared to E. coli (20 mm at 100 mg/ml). Statistical analysis (ANOVA) confirmed concentration-dependent efficacy (p < 0.001). The findings suggest that Aloe vera extract possesses potent antibacterial properties, supporting its potential as a natural alternative to conventional antibiotics. Further research is recommended to isolate active compounds, conduct clinical trials, and explore synergistic effects with existing antibiotics to combat antibiotic resistance. Sustainable production methods and public awareness on medicinal plant usage are also advocated.