ANTIFUNGAL ANALYSIS OF THE LEAF OF MORINGA OLEIFERA FOR THE CONTROL OF FUSARIUM AND ALTERNARIA AFFECTING LYCOPERSICUM SP AND DAUCUS CAROTA (L)
Abstract
Fungal infections significantly reduce the yield and quality of tomato (Lycopersicum sp.) and
carrot (Daucus carota L.), posing a challenge to sustainable agriculture. This study aimed to
evaluate the antifungal activity of Moringa oleifera leaf extracts as a potential biocontrol agent
against Fusarium and Alternaria species infecting these crops. Fresh Moringa leaves were
collected, dried, and extracted using ethanol and methanol. Fungal pathogens were isolated from
diseased plant samples, identified morphologically, and subjected to antimicrobial assays using
the agar well diffusion method, minimum inhibitory concentration (MIC), and minimum
fungicidal concentration (MFC). Results revealed that ethanol extracts exhibited significant
antifungal activity, with inhibition zones of 20 mm and 18 mm against Fusarium and Alternaria
respectively at 100 mg/ml. MIC and MFC values were determined at 12.5 mg/ml for both fungi,
indicating strong fungistatic and fungicidal properties. In contrast, methanol extracts showed no
antifungal activity across all concentrations. These findings suggest ethanol is a more effective
solvent for extracting antifungal compounds from Moringa leaves.The study concludes that
Moringa oleifera ethanol extracts hold promise as a natural, eco-friendly alternative to synthetic
fungicides for managing fungal pathogens in tomato and carrot cultivation. It is recommended
that further phytochemical analysis be conducted to isolate active compounds, alongside field
trials and toxicological assessments, to validate practical applications in crop protection.
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