COMPARATIVE EVALUATION OF THE PHENOLIC CONTENT OF N -HEXANE LEAF AND AQUEOUS EXTRACTS OF MONODORA MYRISTICA
Abstract
ABSTRACT
This study investigated the phytochemical composition and phenolic content of Monodora myristica extracts using aqueous and N-hexane solvents. Qualitative phytochemical screening revealed that the aqueous extract contained mainly polar compounds, including alkaloids, flavonoids, tannins, phenols, saponins, and glycosides, while the N-hexane extract contained non-polar metabolites such as steroids and terpenoids, alongside alkaloids, saponins, and glycosides. Quantitative analysis of phenolic content was performed using a standard calibration curve and absorbance measurements. Results showed that the N-hexane extract exhibited significantly higher phenolic content (922.5–3064.5 µg AAE/mL) compared to the aqueous extract (154.5–562.5 µg AAE/mL), indicating the influence of solvent polarity on extraction efficiency. The comparative bar chart and calibration graph confirmed these findings, demonstrating that N-hexane effectively extracts lipophilic phenolics, while aqueous solvent favors hydrophilic compounds. Overall, the study confirms that Monodora myristica is a rich source of bioactive compounds with potential antioxidant, antimicrobial, and medicinal applications. The findings highlight the importance of solvent selection in phytochemical studies and suggest that N-hexane extracts may offer stronger bioactivity for future pharmacological investigations.
Full-Text Access Notice
In accordance with the NERD Policy on promoting peer-reviewed publication, public access to the full text of a project, thesis or dissertation is restricted for three years, allowing the author and supervisors sufficient time to pursue peer-reviewed publication.
During this period, researchers with legitimate academic or research purposes may request authorisation directly from the author to enable NERD to release the indexed full texts of the work using the form below.