PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL SCREENING OF AZADIRACHTA INDICA LEAVES AND STEM BARK EXTRACTS

Study was aimed to determine the phytochemical constituents and in vitro Antibacterial activity of Ethanol, Chloroform, Methanol, and n-hexane extracts from Azadirachta indica Leaves and Stem Bark against Pseudomonas aeruginosa and Streptococus puemonia. The Phytochemical screening of the Plant Materials for various phytochemical constituents was conducted using Qualitative method. The sensitivity of each extracts against the isolates was determined using the Agar well diffusion and broth dilution method was employed for the determination of Minimum Inhibitory concentration (MIC) and Minimum Bactericidal concentration (MCB) of the extracts. The result of preliminary Phytochemical screening of the extracts showed the presence of Alkaloids, Flavonoids, Saponins, Steroids, Terpenoids, Glycosides, Tannis, and Anthraquinones. The Antibacterial activity of the extracts showed that the extracts were active against the isolates with Streptococus puemonia being the most susceptible isolate with the highest average zoneof inhibition of 20.1 mg/ml and Pseudomonas aeruginosa the least susceptible with the highest average zone of inhibition of 18.9 mg/ml. Statistical analysis of the result showed that Chloroform extract is more effective than Ethanol extract while the Leaves of the Plant showed higher efficiency than the Stem Bark. However, Finding of the study justify the Antibacterial efficiency of the Leaves and Stem Bark of Azadirachta indica.